Variables affecting resolution of lung phospholipids in one-dimensional thin-layer chromatography.

Separation of phosphatidylinositols and other phospholipids by two-step one-dimensional thin-layer chromatography.

A quick and efficient thin-layer chromatographic procedure is described for the separation of phosphatidylinositol-4,5-bisphosphate, phosphatidylinositol-4-monophosphate, phosphatidylinositol, phosphatidylcholine, phosphatidic acid, and phosphatidylethanolamine. The method involves development of the phospholipids successively in two different solvent systems but in the same direction. The meth...

Estimation of phosphatidylglycerol in fluids containing pulmonary surfactant.

A simple, direct and non-destructive method for quantitative separation of phosphatidylglycerol from other phospholipids in pulmonary washings is described. Total lipid extracts from dog lung washings and phosphatidylglycerol standard were spotted quantitatively on chromatoplates and separated by one-dimensional thin-layer chromatography in chloroform-methanol-water 65:25:4 (v/v/v). Quantificat...

Rapid quantitative measurement of lung tissue phospholipids.

A rapid procedure for the separation of phospholipids of lung tissue into acidic and nonacidic fractions by means of diethylaminoethyl cellulose acetate microcolumns is described. The fractions are then resolved into individual phospholipids by thin-layer chromatography and quantified by transmission densitometry.

Quantitative Two-dimensional Thin-layer Chromatography of Naturally Occurring Phospholipids.

Unidimensional thin-layer chromatography of phospholipids on boric acid-impregnated plates.

A unidimensional thin-layer chromatography system for the separation of phospholipids has been developed. The method employs precoated plates impregnated with boric acid and is capable of resolving eight phospholipid standards, including phosphatidylinositol, phosphatidylserine, and phosphatidylglycerol. Liver, kidney, and platelet phospholipids of the rat were resolved into six major classes u...